Coding

Part:BBa_K4842006

Designed by: Ren Yiyang   Group: iGEM23_WestCentral-HS   (2023-10-07)


pnar-3

Despite some exciting achievements in the heterologous biosynthesis of (S)-estragenol, heterologous expression of this pathway in E. coli currently relies on isopropyl-β-d-thiogalactopyranoside (IPTG) induction, and the high price and toxicity of IPTG to E. coli cells have limited the application of this induced expression on an industrial scale. The dissolved oxygen (DO)-dependent nar promoter is considered an alternative to the above promoters because of its relatively simple and cost-effective induction mechanism and function at any stage of cell growth.Based on the original promoter, we have optimized its RBS sequence and plan to experimentally screen for sequences that can increase protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 207
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 207
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 207
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None